Some of the methods we use:
PCR for direct and random mutagenesis, cloning of reporter stains etc.
Real-time PCR
RNA seq (RNA sequencing)
Western Blot analysis
Co-IP
Confocal microscopy
Fluorescence-lifetime imaging microscopy (FLIM)
Electron microscopy
Flow cytometry and Fluorescence-activated cell sorting (FACS)
PCR for direct and random mutagenesis, cloning of reporter stains etc.
Related to: http://www.ncbi.nlm.nih.gov/pubmed/24798071
Real-time PCR
RT-qPCR analyses is employed for comparison of transcript levels in different strains
Related to: https://www.ncbi.nlm.nih.gov/pubmed/27558743
RNA seq (RNA sequencing)
Deep RNA sequencing is used to compare the global transcriptomes of a planktonic and biofilm- forming strains of the cyanobacterium Synechococcus elogatus.
mRNA profiles of the gene Synpcc7942_1134 (unpublished).
Western Blot analysis
Western blot analysis using anti-GFP to examine the stability of free GFP versus that of NblA::GFP.
Related to: http://www.ncbi.nlm.nih.gov/pubmed/24798071
Co-IP
To investigate the cellular role of particular proteins we use co-immunoprecipitation to identify targets of interaction. Analysis by mass spectrometry is used to identify the co-precipitating proteins.
Confocal microscopy
Cell imaging by fluorescence microscopy
Related to: https://www.ncbi.nlm.nih.gov/pubmed/24798071
Fluorescence-lifetime imaging microscopy (FLIM)
FLIM is used to examine the interaction between proteins
Related to: https://www.ncbi.nlm.nih.gov/pubmed/26173720
Electron microscopy
Transmission electron microscope (TEM)
Cryo scanning electron microscopy (cryo-SEM)
Cryo-SEM was used to characterize biofilms of the T2SE-mutant of Synechococcus elongatus (A and B) and wild type cells (C).
Related to: https://www.ncbi.nlm.nih.gov/pubmed/23298171
Flow cytometry and Fluorescence-activated cell sorting (FACS)
Flow cytometric analysis of Sytox treated cells inoculated into fresh medium (FM) or conditioned medium (CM).
Related to: https://www.ncbi.nlm.nih.gov/pubmed/24959874
