What methods do we use?

What methods do we use?

Some of the methods we use:

PCR for direct and random mutagenesis, cloning of reporter stains etc.
Real-time PCR
RNA seq (RNA sequencing)
Western Blot analysis
Co-IP
Confocal microscopy
Fluorescence-lifetime imaging microscopy (FLIM)
Electron microscopy
Flow cytometry and Fluorescence-activated cell sorting (FACS)  

 

 

PCR for direct and random mutagenesis, cloning of reporter stains etc.

Related to: http://www.ncbi.nlm.nih.gov/pubmed/24798071

 


 

 

 

 

 

Real-time PCR

RT-qPCR analyses is employed for comparison of transcript levels in different strains  

Related to: https://www.ncbi.nlm.nih.gov/pubmed/27558743


 

 

 

RNA seq (RNA sequencing)

Deep RNA sequencing is used to compare the global transcriptomes of a planktonic and biofilm- forming strains of the cyanobacterium Synechococcus elogatus.

mRNA profiles of the gene Synpcc7942_1134 (unpublished).


 

 

 

Western Blot analysis

Western blot analysis using anti-GFP to examine the stability of free GFP versus that of NblA::GFP.

Related to: http://www.ncbi.nlm.nih.gov/pubmed/24798071


 

 

 

Co-IP

To investigate the cellular role of particular proteins we use co-immunoprecipitation to identify targets of interaction. Analysis by mass spectrometry is used to identify the co-precipitating proteins.

 

 

 

Confocal microscopy

Cell imaging by fluorescence microscopy

Related to: https://www.ncbi.nlm.nih.gov/pubmed/24798071


 

 

 

Fluorescence-lifetime imaging microscopy (FLIM)

FLIM is used to examine the interaction between proteins

Related to: https://www.ncbi.nlm.nih.gov/pubmed/26173720


 

 

 

Electron microscopy

Transmission electron microscope (TEM)

Cryo scanning electron microscopy (cryo-SEM)

Cryo-SEM was used to characterize biofilms of the T2SE-mutant of Synechococcus elongatus (A and B) and wild type cells (C).

Related to: https://www.ncbi.nlm.nih.gov/pubmed/23298171

 


 

 

 

 

Flow cytometry and Fluorescence-activated cell sorting (FACS)

Flow cytometric analysis of Sytox treated cells inoculated into fresh medium (FM) or conditioned medium (CM).

Related to: https://www.ncbi.nlm.nih.gov/pubmed/24959874

 

 

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Prof. Rakefet Schwarz Laboratory

The Mina & Everard Goodman Faculty of Life Sciences